Techniques in Confocal Microscopy,, P. Michael Conn
Автор: Robert Price; Jay Jerome (Eds.) Название: Basic Confocal Microscopy ISBN: 0387781749 ISBN-13(EAN): 9780387781747 Издательство: Springer Рейтинг: Цена: 174150.00 T Наличие на складе: Поставка под заказ. Описание: Most researchers agree that biological confocal microscopy was jump-started by the confocal design first published by White and Amos in 1985 in the Journal of Cell Biology. As a result, this remains a relatively young field. Yet the use of the technique has grown phenomenally since those early efforts, with new users joining the ranks daily. The publication of Basic Confocal Microscopy reflects the burgeoning need to train new students, technologists, and faculty wishing to use confocal microscopy in their research. A direct outgrowth of the authors’ five-day intensive course in the subject begun in 2005, this book covers the basics and includes all the information required to design, implement, and interpret the results of, biological experiments based on confocal microscopy. Concise yet comprehensive, the volume begins by covering the core issues of fluorescence, specimen preparation and labeling, before moving on to address the analog-to-digital conversion of specimen data gathered using confocal microscopy. Subsequent chapters detail the practicalities of operating confocal microscopes, providing all the information necessary to begin practicing confocal microscopy as well as optimizing the material obtained. The final block of chapters examine 3-dimensional analysis and the reconstruction of data sets, outline some of the ethical considerations in confocal imaging, and then supply a number of resources that the authors have found useful in their own work. Once readers have mastered the information this book presents, the resources found in its pages will be an excellent guide to continued learning about the more advanced forms of confocal microscopy.
Автор: Alberto Diaspro Название: Confocal and Two-Photon Microscopy: Foundations, Applications and Advances ISBN: 0471409200 ISBN-13(EAN): 9780471409205 Издательство: Wiley Рейтинг: Цена: 261830.00 T Наличие на складе: Поставка под заказ. Описание: Confocal and two-photon fluorescence microscopy has provided researchers with unique possibilities of three-dimensional imaging in media like human tissue or semiconductor integrated circuits.
Автор: Pawley Название: Handbook of Biological Confocal Microscopy ISBN: 038725921X ISBN-13(EAN): 9780387259215 Издательство: Springer Рейтинг: Цена: 186330.00 T Наличие на складе: Невозможна поставка. Описание: This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging.
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