PCR Primer Design, Yuryev Anton

Автор: Najafov Ayaz
Название: PCR Guru
ISBN: 0128042311 ISBN-13(EAN): 9780128042311
Издательство: Elsevier Science
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Цена: 66240.00 T
Наличие на складе: Есть у поставщика Поставка под заказ.
Описание: PCR Guru: An Ultimate Benchtop Reference for Molecular Biologists is written with an aim of providing researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with PCR setup and optimization. As a laboratory guide, it emphasizes the technical aspects of employing PCR as a tool in molecular biology laboratories. PCR Guru covers the history of PCR and the basic science underlying it and discusses what PCR is on the bench level starting with detailed description and tips on primer design and continuing with what are the standard protocols used to perform PCR.

• Provides troubleshooting tips for various types of modifications of standard protocols
• Contains unique "Good Practices and Tips┐ that are indispensable for the beginner and expert alike
• Features "Special Cases┐ with applications of PCR, optimization, and troubleshooting
• Includes detailed appendices with tables, figures, and key protocols
• Organized as a systematic, concentrated resource to save time when addressing a PCR problem

Автор: Connell Joe
Название: RT-PCR Protocols
ISBN: 0896038750 ISBN-13(EAN): 9780896038752
Издательство: Springer
Цена: 88470.00 T
Наличие на складе: Поставка под заказ.
Описание: A panel of highly regarded molecular biologists and clinical researchers describe in detail their most novel, useful, and interesting RT-PCR applications. Here the newcomer will find readily reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in tissue, and the cloning of genes, as well as for analyzing T-cell clones and the differential expression of genes. For the expert seeking to extend the usefulness of RT-PCR, there are user-friendly applications that complement the latest technological advances, including laser-capture microdissection (LCM), real-time and quantitative PCR, microarray technology, cDNA cloning, and antibody engineering.