Protein Engineering Protocols, Müller Kristian, Arndt Katja
Àâòîð: Robert Lanza Íàçâàíèå: Principles of Tissue Engineering, ISBN: 0123983584 ISBN-13(EAN): 9780123983589 Èçäàòåëüñòâî: Elsevier Science Ðåéòèíã: Öåíà: 130670.00 T Íàëè÷èå íà ñêëàäå: Åñòü Îïèñàíèå: Now in its fourth edition, Principles of Tissue Engineering has been the definite resource in the field of tissue engineering for more than a decade. The fourth edition provides an update on this rapidly progressing field, combining the prerequisites for a general understanding of tissue growth and development, the tools and theoretical information needed to design tissues and organs, as well as a presentation by the world’s experts of what is currently known about each specific organ system. As in previous editions, this book creates a comprehensive work that strikes a balance among the diversity of subjects that are related to tissue engineering, including biology, chemistry, material science, and engineering, among others, while also emphasizing those research areas that are likely to be of clinical value in the future. . This edition includes greatly expanded focus on stem cells, including induced pluripotent stem (iPS) cells, stem cell niches, and blood components from stem cells. This research has already produced applications in disease modeling, toxicity testing, drug development, and clinical therapies. This up-to-date coverage of stem cell biology and other emerging technologies –such as brain-machine interfaces for controlling bionics and neuroprostheses– is complemented by a series of new and updated chapters on recent clinical experience in applying tissue engineering, as well as a new section on the application of tissue-engineering techniques for food production. The result is a comprehensive textbook that will be useful to students and experts alike.
Àâòîð: Armstrong Donald Íàçâàíèå: Oxidative Stress Biomarkers and Antioxidant Protocols ISBN: 0896038505 ISBN-13(EAN): 9780896038509 Èçäàòåëüñòâî: Springer Ðåéòèíã: Öåíà: 144410.00 T Íàëè÷èå íà ñêëàäå: Åñòü ó ïîñòàâùèêà Ïîñòàâêà ïîä çàêàç. Îïèñàíèå: Donald Armstrong updates and expands his highly praised Free Radical and Antioxidant Protocols (Humana, 1998) with a collection of new and valuable methods for evaluating the pertubations in cell function resulting from increased oxidative stress. Presented in a user-friendly, step-by-step format, these readily reproducible techniques cover both free radical-derived and antioxidant biomarkers. The methodologies demonstrated include ELISA, HPLC, infrared spectroscopy, gas chromatography-mass spectroscopy, immunoblotting, electroelution fractionation, isoelectric focusing, voltametry, and electron paramagnetic resonance imaging. Special emphasis is given to the separation of complex mixtures of plant antioxidants, soft drug design to protect from toxic oxidative metabolites, in vitro oxidation conditions, and correcting for random measurement error to improve statistical interpretation. A soon-to-be-published companion volume, Oxidants and Antioxidants: Ultrastructural and Molecular Biology Protocols (0-89603-851-3), contains state-of-the-art molecular and ultrastructural methods that expand the total number of protocols to 109 assays.
Àâòîð: Lo Benny K. C. Íàçâàíèå: Antibody Engineering / Methods and Protocols ISBN: 1588290921 ISBN-13(EAN): 9781588290922 Èçäàòåëüñòâî: Springer Ðåéòèíã: Öåíà: 163040.00 T Íàëè÷èå íà ñêëàäå: Åñòü ó ïîñòàâùèêà Ïîñòàâêà ïîä çàêàç. Îïèñàíèå: A core collection of diverse cutting-edge techniques for the generation, expression, optimization, and characterization of recombinant antibodies. Readily reproducible protocols for lead generation range from the cloning of human immunoglobulin genes to the selection and generation of human recombinant antibodies by humanization approaches, molecular display technologies and transgenic animals. Procedures are also described on restructuring antibody leads into monovalent, multivalent, and bispecific binding fragments for a wide variety of in vivo applications. State-of-the-art technologies are described for the characterization of antigen-binding affinity and specificity with novel applications in radioimmunotargeting, cancer immunotherapy, drug abuse, and proteomics.
Àâòîð: Federico Maurizio Íàçâàíèå: Lentivirus Gene Engineering Protocols ISBN: 1607615320 ISBN-13(EAN): 9781607615323 Èçäàòåëüñòâî: Springer Ðåéòèíã: Öåíà: 111790.00 T Íàëè÷èå íà ñêëàäå: Åñòü ó ïîñòàâùèêà Ïîñòàâêà ïîä çàêàç. Îïèñàíèå: Authoritative and timely, Lentivirus Gene Engineering Protocols, Second Edition, covers the most relevant issues and techniques of LV-based gene engineering. This practical guide is aimed at those who wish to know more about this vital area of study.
Àâòîð: Krishna Mohan Poluri; Khushboo Gulati Íàçâàíèå: Protein Engineering Techniques ISBN: 9811027315 ISBN-13(EAN): 9789811027314 Èçäàòåëüñòâî: Springer Ðåéòèíã: Öåíà: 55890.00 T Íàëè÷èå íà ñêëàäå: Åñòü ó ïîñòàâùèêà Ïîñòàâêà ïîä çàêàç. Îïèñàíèå: This brief provides a broad overview of protein-engineering research, offering a glimpse of the most common experimental methods. It also presents various computational programs with applications that are widely used in directed evolution, computational and de novo protein design. Further, it sheds light on the advantages and pitfalls of existing methodologies and future perspectives of protein engineering techniques.
